A rapid and sensitive enzymatic-radiochemical assay for the determination of triglycerides.
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A rapid and sensitive enzymatic-radiochemical assay for the determination of triglycerides.
An enzymatic-radiochemical method suitable for the determination of triglyceride levels of cells in culture is described. The method is based on the enzymatic hydrolysis of triglycerides to free fatty acids which then complex with 63Ni. The method is rapid, accurate, and inexpensive. The procedure extends the sensitivity of triglyceride measurement to as low as 0.25 nanomoles.
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ژورنال
عنوان ژورنال: Journal of Lipid Research
سال: 1987
ISSN: 0022-2275
DOI: 10.1016/s0022-2275(20)38654-5